Mutational studies and engineering of active site residues
Mutants, P172L, and P172L/F250A showed enhanced / and values by 5.3- and 6.9-fold Also, mutant L167W had the best synergism with T. reesei in cellulosic biomass degradation
cel3a from Talaromyces emersonii was expressed in T. reesei
High specific activity against p-nitrophenyl-β-D-glucopyranoside (, 512 IU/mg) and was competitively inhibited by glucose (ki, 0.254 mM) and displayed transferase activity
0.02% Tween-80 concentration was optimum, pH 5.0 and temperature (31°C) initially (for 18 h) was optimum for maximum production of cellulase and β-glucosidase