208912.fig.001a
(a)
208912.fig.001b
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208912.fig.001c
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Figure 1: Formation of lymphedema in the right hindlimb of Balb/c mice. (a) Meticulous dissection and preservation of the neurovascular bundle by gentle retraction. (b) Circumferential resection of strip of skin, subcutaneous tissue, and muscle to obliterate deep lymphatics. (c) Staining of lymph nodes and lymphatics after injection of methylene blue (black arrow) for lymphatic obliteration and lymph node dissection.