208912.fig.004a
(a)
208912.fig.004b
(b)
Figure 4: Upregulation of lymphatic specific genes after coculture of MDSCs with the supernatant of HLECs. (a) RT-PCR demonstrates negative expression of genes for Prox-1, FLt-4, and podoplanin in native MDSCs, while the coculture demonstrates upregulation of these genes. (b) Graphical representation of gene expression rate normalized against actin. There is upregulation of genes for Prox-1, FLt-4, and podoplanin after coculture compared to native MDSCs.