Research Article
Plasma and Ocular Prednisolone Disposition after Oral Treatment in Cats
Table 1
HPLC analytical validation.
| | Chromatographic parameter | Plasma | Aqueous humor |
| | Absolute recovery range (%) | 72–78 | 89–93 | | Intraday precision (%CV) | 3.2–5.5 | 3.3–4 | | Interday precision (%CV) | 4.2–7.6 | 2.3–11.1 | | Interday accuracy (%RE) | 5.1–13.1 | 7.0–17.2 | | LOD (ng/mL) | 4.3 | 1.3 | | LOQ (ng/mL) | 25 | 5 | | Precision LOQ (%CV) | 12.4 | 5.8 | | Stability (%CV) | 3.8–4.5 | 0.9–4.6 |
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CV: coefficient of variation; LOD: limit of detection; LOQ: limit of quantification; RE (relative error) = 100 [(predicted concentration − nominal concentration)/nominal concentration].
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