Magnetofection: A Reproducible Method for Gene Delivery to Melanoma Cells
Figure 3
The effect of different SPIONs’ synthesis on magnetofection efficacy in murine B16F1 melanoma cells. (a) The expression of eGFP in murine B16F1 melanoma cells visualized by fluorescence microscopy. The images were taken 24 h after magnetofection under ×60 magnification. For magnetofection SPIONs-PAA-PEI-pDNA complexes were prepared using SPIONs from all synthesis batches (from MF_S1 to MF_S6). The first row of images represents cells under visible light (VIS) and the second under fluorescent light (FLU). Scale bar, 200 μm. (b) The expression of eGFP in murine B16F1 melanoma cells quantified by flow cytometry. The results were obtained 24 h after magnetofection with SPIONs-PAA-PEI-pDNA complexes prepared from different SPIONs’ synthesis batches (from MF_S1 to MF_S6). Bars and squares represent AM and SEM of the percentage of fluorescent cells and the median fluorescence intensity, respectively.
