Magnetofection: A Reproducible Method for Gene Delivery to Melanoma Cells
Figure 4
The effect of PEI water solution preparation on magnetofection efficacy in murine B16F1 melanoma cells. (a) The expression of eGFP in murine B16F1 melanoma cells visualized by fluorescence microscopy. The images under ×60 magnification were taken 24 h after transfection with PEI and pDNA complexes (from PEI-pDNA_P1 to PEI-pDNA_P8) and magnetofection (from MF_P1 to MF_P8) using eight different PEI water solutions. The first and the third row of images represent cells under visible light (VIS) and the second and the fourth row represent cells under fluorescent light (FLU). Scale bar, 200 μm. (b) The expression of eGFP in murine B16F1 melanoma cells quantified by flow cytometry. The results were obtained 24 h after transfection with PEI and pDNA complexes (from PEI-pDNA_P1 to PEI-pDNA_P8) and magnetofection (from MF_P1 to MF_P8) using different PEI water solutions. Bars and squares represent AM with SEM of the percentage of fluorescent cells and the median of fluorescence intensity, respectively. Asterisks indicate statistically significant differences between percentages of fluorescent cells after transfection or magnetofection, while circles denote the differences in the median fluorescence intensities after transfection or magnetofection ().
