About this Journal Submit a Manuscript Table of Contents
BioMed Research International
Volume 2013 (2013), Article ID 214864, 10 pages
http://dx.doi.org/10.1155/2013/214864
Research Article

Expression of Syndecan-4 and Correlation with Metastatic Potential in Testicular Germ Cell Tumours

1Hematology Division, Department of Internal Medicine, University Hospital of Patras, 26500 Patras, Greece
2Clinical Oncology Laboratory, Division of Oncology, Department of Medicine, University Hospital of Patras, 26500 Patras, Greece
3Laboratory of Biochemistry, Department of Chemistry, University of Patras, 26500 Patras, Greece
4Department of Pathology, University Hospital of Patras, 26500 Patras, Greece
5Division of Urology, Department of Medicine, University Hospital of Patras, 26500 Patras, Greece

Received 30 April 2013; Accepted 28 May 2013

Academic Editor: Martin Götte

Copyright © 2013 Vassiliki T. Labropoulou et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Although syndecan-4 is implicated in cancer progression, there is no information for its role in testicular germ cell tumours (TGCTs). Thus, we examined the expression of syndecan-4 in patients with TGCTs and its correlation with the clinicopathological findings. Immunohistochemical staining in 71 tissue specimens and mRNA analysis revealed significant overexpression of syndecan-4 in TGCTs. In seminomas, high percentage of tumour cells exhibited membranous and/or cytoplasmic staining for syndecan-4 in all cases. Stromal staining for syndecan-4 was found in seminomas and it was associated with nodal metastasis ( ), vascular/lymphatic invasion ( ), and disease stage ( ). Reduced tumour cell associated staining for syndecan-4 was observed in nonseminomatous germ cell tumours (NSGCTs) compared to seminomas. This loss of syndecan-4 was associated with nodal metastasis ( ), vascular/lymphatic invasion ( ), and disease stage ( ). Stromal staining for syndecan-4 in NSGCTs did not correlate with any of the clinicopathological variables. The stromal expression of syndecan-4 in TGCTs was correlated with microvessel density ( ). Our results indicate that syndecan-4 is differentially expressed in seminomas and NSGCTs and might be a useful marker. Stromal staining in seminomas and reduced levels of syndecan-4 in tumour cells in NSGCTs are related to metastatic potential, whereas stromal staining in TGCTs is associated with neovascularization.