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Figure 3: TLR4 expression in L. mexicana-infected macrophages. Cells were treated or not with PPAR agonists for 24 h and cPLA2 antagonist for 1 h before infection. TLR4 expression was analyzed by flow cytometry. LPS (2 h) was used as a positive control of induction. Graph bars are mean ± SEM of three independent experiments, and statistical analysis was performed comparing, for each time, treated versus non-treated macrophages; (*) , (**) , and (***) .