miR-1-Mediated Induction of Cardiogenesis in Mesenchymal Stem Cells via Downregulation of Hes-1

Figure 2

Western blot was performed for Notch signaling and cardiomyocyte-specific markers in MSCs, MSCs^null, and MSCs^miR-1 (1 d, 7 d, and 14 d). (a) Expression of Notch-1, Notch-2, Notch-4, Dll-1, Dll-4, Jag-1, Hes-1, and Hey-1 were detected on MSCs. Semiquantitative data showed that the ratio of optical density for Notch-1, Notch-2, Notch-4, Dll-1, Dll-4, Jag-1, and Hey-1 did not alter in MSCs^miR-1 on days 1, 7, and 14 (b)–(h). The expression of Hes-1 (i) in MSCs^miR-1 was decreased by days 7 and 14. In MSCs^miR-1, the expression of Nkx2.5 (j) and GATA-4 (k) were detected on day 7 and decreased by day 14. cTnT (l) and CX43 (m) expression were detected on day 7 and significantly increased by day 14 (control = MSCs; null = MSCs^null = MSCs infected with mock lentiviral vectors without miR-1; miR-1= MSCs^miR-1 = MSCs infected with miR-1 recombinant lentiviral vectors; compared to MSCs, ^∗𝑃<0.05, ^★P < 0.01; compared to MSCs^null, ^#𝑃<0.05, ^※P < 0.01; compared to MSCs^miR-1 (1 d), ^&𝑃<0.05, ^▲𝑃<0.01; compared to MSCs^miR-1 (7 d), ^@𝑃<0.05).

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