Differentiation of Mesenchymal Stem Cells from Human Umbilical Cord Tissue into Odontoblast-Like Cells Using the Conditioned Medium of Tooth Germ Cells In Vitro
Figure 1
Isolative process and morphological appearance of mesenchymal stem cells (MSCs) isolated from human umbilical cords (hUCMSCs). (a) The umbilical cord (UC) was cut into pieces of approximately 4 to 5 cm in length. (b) Each segment of the UC was dissected longitudinally to expose the Wharton’s jelly (WJ). (c) WJ was obtained by removing the cord vessels and the amniotic epithelium. (d) The appearance of primary hUCMSCs cultured for 5 days. (e) The appearance of primary hUCMSCs cultured for 12 days. (f) After 19 days of culture, the primary hUCMSCs reached 80–90% confluency. (g) The appearance of hUCMSCs after passaging. Scale bar = 100 μm.
