Differentiation of Mesenchymal Stem Cells from Human Umbilical Cord Tissue into Odontoblast-Like Cells Using the Conditioned Medium of Tooth Germ Cells In Vitro
Figure 5
Detecting results of odontogenic differentiation of hUCMSCs induced in TGC-CM. (a) Human UCMSCs induced by TGC-CM were positively stained for DSP, but the control cells were not. Scale bar = 100 μm. (b) Human UCMSCs cultured in TGC-CM were positively stained for DMP1, but the control cells were not. Scale bar = 100 μm. (c) The reverse transcription PCR products indicated the expression of DSPP and DMP1 mRNA in the TGC-CM-treated UCMSCs. β-actin was used as an internal control for each group.
