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BioMed Research International
Volume 2013 (2013), Article ID 218593, 3 pages
http://dx.doi.org/10.1155/2013/218593
Methodology Report

Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification

1Centre for Malaria and Tropical Diseases, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira, 100, 1349-008 Lisboa, Portugal
2Unidad de Biofísica (UBF), CSIC-UPV/EHU, Barrio Sarriena S/N, Bizkaia, 48940 Leioa, Spain
3Centro de Estudios Parasitológicos y de Vectores (CEPAVE), CCT-La Plata/CONICET-UNLP, No. 584, 1900 La Plata, Argentina

Received 10 January 2013; Revised 15 March 2013; Accepted 21 March 2013

Academic Editor: Zhirong Sun

Copyright © 2013 Jailson F. B. Querido et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines. The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.