An Inherited Heteroplasmic Mutation in Mitochondrial Gene COI in a Patient with Prostate Cancer Alters Reactive Oxygen, Reactive Nitrogen and Proliferation
Figure 1
Detection of heteroplasmic point mutation of mitochondrial cytochrome oxidase subunit I (COI) mitochondrial gene from a single individual with prostate cancer. (a) Sequencing chromatograms of prostatic tissue and an Epstein-Barr transformed lymphoblast cell line show approximately equal levels of both the wild type (T) and mutant (C) DNA base. (b) Activity of cytochrome oxidase measured in isolated mitochondria prepared from the patient’s heteroplasmic lymphoblasts (see Section 2 for details) compared to the average of two-lymphoblast lines from controls with only the wild type base at position 6124. (c) Flow cytometric analysis of DCF fluorescence in the patient’s heteroplasmic lymphoblasts compared to the average of two-lymphoblast lines from controls with only the wild type base at position 6124 (gray bars). Cells were also analyzed for DCF fluorescence in the presence of FCCP (white bars). Error bars represent the standard deviation of 2–4 data points.
