The Escherichia coli-Derived Thymosin β4 Concatemer Promotes Cell Proliferation and Healing Wound in Mice
Figure 3
(a) Purification of the E. coli-derived 4 × Tβ4. Lane M: middle molecular mass protein markers; lane 1: unbound proteins were eluted using 1×Ni-NTA buffer B; lane 2: nonspecific proteins were eluted using 1 × Ni-NTA buffer C; lanes from 3 to 9: 4 × Tβ4 was eluted using 1 × Ni-NTA buffer E. Red arrows indicate the specific band of E. coli-derived 4 × Tβ4 on SDS/PAGE gel. (b) Assay of mice splenic lymphocyte proliferation using MTT method. 4 × Tβ4: purified 4 × Tβ4 protein extracted from BL21 (E. coli) stimulate, mice splenic lymphocyte proliferation; Tβ4: commercial Tβ4 protein (GL Biochem) stimulates mice splenic lymphocyte proliferation.
