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BioMed Research International
Volume 2013 (2013), Article ID 241780, 11 pages
http://dx.doi.org/10.1155/2013/241780
Research Article

Circulating microRNAs and Kallikreins before and after Radical Prostatectomy: Are They Really Prostate Cancer Markers?

1Department of Medical-Surgical Sciences and Public Health, Faculty of Medicine and Surgery, University of Perugia, Loc. S. Andrea delle Fratte, 06156 Perugia, Italy
2Department of Biopathological Science and Hygiene of Food and Animal Production, Faculty of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06126 Perugia, Italy

Received 10 May 2013; Revised 9 August 2013; Accepted 23 August 2013

Academic Editor: Romonia Renee Reams

Copyright © 2013 Maria Giulia Egidi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of our study was to monitor serum levels of two miRNAs (miR-21 and miR-141) and three KLKs (hK3/PSA, hK11, and hK13) before and 1, 5, and 30 days after radical prostatectomy, in order to characterize their fluctuations after surgery. 38 patients with prostate cancer were included. miR-21 and miR-141 were quantified through real-time PCR, while ELISA assays were used to quantify hK3 (PSA), hK11, and hK13. Both miR-21 and miR-141 showed a significant increase at the 5th postoperative day, after which a gradual return to the preoperative levels was recorded. These findings suggest that miR-21 and miR-141 could be involved in postsurgical inflammatory processes and that radical prostatectomy does not seem to alter their circulating levels. Postoperative serum kallikreins showed a significant decrease, highlighting the potential usefulness of kallikreins apart from PSA as potential prostate cancer markers.