Research Article
Polymorphism of the Transferrin Gene in Eye Diseases: Keratoconus and Fuchs Endothelial Corneal Dystrophy
Table 2
Data for PCR-RFLP analysis used to genotype polymorphisms in the TF gene.
| | Polymorphism | Primers sequences (forward, reverse) | Annealing temperatures in PCR (°C) | Restriction enzymes (recognized allele) | Fragments
(bp) |
| g.3296G>A
rs8177178 | AGGGCATAGAGCTGGCTGCT
GAAGACTGTTAGCATGAGGGCC | 66.2 | RsaI (A) | GG 483
GA 483/327/156
AA 327/156 | g.3481A>G
rs8177179 | AGCTGTATGTGTGCATGCTGCTC
GGGCCAATTCACACATTCAAT | 60.5 | StyI (A) | AA 274/198
AG 472/274/198
GG 472 | c.–2G>A
rs1130459 | AGAAAATGAGGTGATCAGTGGG ATGGAAAGGCACCCAGACAC | 66.0 | FokI (G) | GG 218/157
GA 375/218/157
AA 375 |
|
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