Table 2: Expression of proinflammatory cytokine mRNA and protein by IL-33-stimulated MH-S cells.

CytokineExpression of mRNA (relative to HPRT × 103)Concentration of protein in culture supernatant
UnstimulatedIL-33 stimulatedUnstimulatedIL-33 stimulated

TNF- 3.43 ± 0.336.72 ± 0.86**197.3 ± 38.2398.3 ± 23.8**
IL-1 0.66 ± 0.043.88 ± 0.48***51.6 ± 28.493.1 ± 9.7
IL-69.9 ± 6.285.5 ± 9.5***124.0 ± 8.5709.7 ± 38.8***
CXCL-10.001 ± 0.0000.002 ± 0.000**10.6 ± 0.419.2 ± 0.6***
IL-12p400.003 ± 0.0000.228 ± 0.004***11.2 ± 1.449.2 ± 0.6***
G-CSF0.001 ± 0.0000.004 ± 0.00187.9 ± 8.5538.5 ± 33.2***

Data are mean ± SEM ( 4–6 replicates per group). Significant differences relative to unstimulated control are shown as **( ) and ***( ).