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BioMed Research International
Volume 2013 (2013), Article ID 251098, 7 pages
http://dx.doi.org/10.1155/2013/251098
Research Article

Long Noncoding RNA HOTAIR Is Associated with Motility, Invasion, and Metastatic Potential of Metastatic Melanoma

Lihua Tang,1 Wei Zhang,2,3 Bing Su,2,3 and Bo Yu1,3,4,5

1The Affiliated Shenzhen Hospital, Anhui Medical University, Hefei, Anhui 230001, China
2Biomedical Research Institute, Shenzhen PKU-HKUST Medical Center, Shenzhen, Guangdong 518036, China
3Shenzhen Key Lab for Translational Medicine of Dermatology, Shenzhen PKU-HKUST Medical Center, Shenzhen, Guangdong 518036, China
4Shenzhen Key Discipline of Dermatology, Shenzhen Hospital, Peking University, Shenzhen, Guangdong 518036, China
5Department of Dermatology, Shenzhen Hospital, Peking University, Shenzhen, Guangdong 518036, China

Received 22 February 2013; Accepted 21 May 2013

Academic Editor: Marcelo Palma Sircili

Copyright © 2013 Lihua Tang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Metastatic melanoma, the primary cause of skin cancer-related death, warrants new therapeutic approaches that target the regulatory machinery at molecular level. While long noncoding RNAs (lncRNAs) are dysregulated in a number of cancer types, limited data are available on the expression and function of lncRNAs in melanoma metastasis. The primary objective of this study was to investigate the role of 6 metastasis-related lncRNAs in pairs of primary melanoma and matched lymph node metastatic tissues. Among the tested lncRNAs, HOTAIR was the most highly expressed in lymph node metastasis. The role of HOTAIR in melanoma cell motility and invasion was further evaluated by knocking down HOTAIR with siRNAs. Knockdown of HOTAIR resulted in the reduction of motility and invasion of human melanoma cell line A375, as assessed by wound healing assay and Matrigel-based invasion assay. siHOTAIR also suppressed the degradation of gelatin matrix, suggesting that HOTAIR promotes gelatinase activity. Together, our study shows that HOTAIR is overexpressed in metastatic tissue, which is associated with the ability of HOTAIR to promote melanoma cell motility and invasion. These data indicate that lncRNAs may be involved in the metastasis of melanoma and provide support for further evaluation of lncRNAs in melanoma.