A Flow Cytometric Analysis of Vitreous Inflammatory Cells in Patients with Proliferative Diabetic Retinopathy

Table 1

Antibodies for 8-colour immunophenotyping of vitreous and peripheral venous blood samples.

Test tube	FITC	PE	PerCP-Cy5.5	APC	PE-Cy7	APC-Cy7
	mAbs	mAbs	mAbs	mAbs	mAbs	mAbs
1	Kappa*  + CD4*	Lambda*  + CD8*	CD45*	CD14**	CD19**	CD3**

Legend: mAbs: monoclonal antibodies, FITC: fluorescein isothiocyanate, PE: phycoerythrin, PerCP-Cy5.5: peridinin chlorophyll protein-Cy5.5, APC: allophycocyanin, PE-Cy7: phycoerythrin-cyanin 7, APC-Cy7: allophycocyanin-cyanin 7; *5 μL monoclonal antibody was added; **3 μL monoclonal antibody was added.