The Complete Exosome Workflow Solution: From Isolation to Characterization of RNA Cargo

Table 1

RNA representation in exosomes versus parental HeLa cells: top 10 RNA transcripts organized by RNA type. Values for cells and exosome preparations represent mean counts of mapped reads to reference transcripts from three replicate cell lysates. Accession numbers and gene symbols were obtained from NCBI RefSeq annotation.

(a)  

miRBase ID	Cells	miRBase ID	Exosomes
hsa-mir-4279	731.00	hsa-mir-21	2015.33
hsa-mir-1234	505.00	hsa-mir-3160-1	1007.33
hsa-mir-451a	410.33	hsa-mir-4739	894.33
hsa-mir-3665	330.33	hsa-mir-31	827.33
hsa-mir-4449	265.67	hsa-mir-23a	783.67
hsa-mir-3960	240.67	hsa-mir-24-2	644.00
hsa-mir-3160-1	238.00	hsa-mir-1273a	521.67
hsa-mir-1273a	233.67	hsa-mir-30a	439.33
hsa-mir-1273g	195.33	hsa-mir-451a	371.33
hsa-mir-92b	180.00	hsa-mir-1273g	368.00
tRNA ID	Cells	tRNA ID	Exosomes
chr1.trna109-GluCTC	175.33	chr6.trna61-MetCAT	34129.67
chr16.trna34-GlyCCC	147.33	chr1.trna109-GluCTC	15924.33
chr16.trna18-GlyGCC	121.67	chr16.trna18-GlyGCC	5696.67
chr6.trna61-MetCAT	101.00	chr1.trna33-GlyGCC	2354.33
chr17.trna28-CysGCA	86.33	chr16.trna10-LysCTT	2119.67
chr5.trna5-ValAAC	52.67	chr5.trna11-LysCTT	2107.33
chr5.trna11-LysCTT	48.00	chr13.trna3-GluTTC	1987.00
chr1.trna33-GlyGCC	35.67	chr16.trna34-GlyCCC	1928.33
chr17.trna10-GlyTCC	33.00	chr1.trna54-GluCTC	1450.33
chr13.trna3-GluTTC	25.33	chr1.trna124-GlyCCC	1365.00

(b)  

mRNA	Accession	Cells	mRNA	Accession	Exosomes
MTRNR2L2	NM_001190470	4793.67	SUSD2	NM_019601	3172.33
MTRNR2L8	NM_001190702	2646.33	BRWD3	NM_153252	501.00
SETD2	NM_014159	544.67	SENP6	NM_015571	362.67
CUX1	NM_001202543	475.33	FAM59B	NM_001191033	355.33
TUBA1C	NM_032704	475.33	TUBBA4	NM_006087	338.33
FXR1	NM_004860	363.33	QRFPR	NM_198179	313.33
SYT6	NM_001253772	319.00	MDK	NM_001012334	307.00
FUS	NM_001170634	287.33	MTRNR2L2	NM_001190470	302.00
MDK	NM_001012334	238.67	CWC25	NM_017748	266.00
UBE2S	NM_014501	230.33	DUSP13	NM_001007272	232.00
ncRNA	Accession	Cells	ncRNA	Accession	Exosomes
SNORA72	NR_002581	3865.67	RNY5	NR_001571	8482.67
MALAT1	NR_002819	879.67	RNY4	NR_004393	6206.33
RNU1-9	NR_004426	798.00	RN5S3	NR_023365	2343.67
RN5S1	NR_023363	668.67	RNY1	NR_004391	2109.67
RNU2-1	NR_002716	629.00	RNY4P8	NR_033356	2094.33
RN7SL1	NR_002715	581.67	RNY3	NR_004392	1119.33
SNORD3C	NR_006881	531.00	RN7SL2	NR_027260	702.33
VDAC1	NR_036624	437.33	SNORD31	NR_002560	619.33
RN7SK	NR_001445	435.33	RNU1-5	NR_004400	561.67
RN7SL2	NR_027260	425.67	RN7SL1	NR_002715	457.33