The Complete Exosome Workflow Solution: From Isolation to Characterization of RNA Cargo

Table 2

RNA representation in exosomes versus cell-free parental serum: top 10 RNA transcripts organized by RNA type. Values for cell-free serum and exosome preparations represent mean counts of mapped reads to reference transcripts from replicate samples prepared from a single donor. Accession numbers and gene symbols were obtained from NCBI RefSeq annotation.

(a)  

miRBase ID	Serum	miRBase ID	Exosomes
hsa-mir-1246	5222.5	hsa-mir-1246	2145.0
hsa-mir-451	2163.5	hsa-mir-451	1837.0
hsa-mir-2115	77.0	hsa-mir-3934	1444.5
hsa-mir-1281	531.0	hsa-mir-1281	1068.5
hsa-mir-486	337.5	hsa-mir-3180-1	871.0
hsa-mir-16-1	326.0	hsa-mir-4257	851.5
hsa-mir-3180-1	116.5	hsa-mir-181d	687.5
hsa-mir-4257	525.0	hsa-mir-486	593.5
hsa-mir-124-1	136.0	hsa-mir-766	580.5
hsa-mir-3167	293.5	hsa-mir-497	565.0
tRNA ID	Serum	tRNA ID	Exosomes
chr6.trna31-SerGCT	801.0	chr6.trna4-ArgTCG	2071.0
chr12.trna10-AspGTC	718.0	chr6.trna31-SerGCT	1867.0
chr6.trna4-ArgTCG	661.5	chr15.trna4-ArgTCG	1010.0
chr15.trna4-ArgTCG	475.0	chr6.trna14-TyrGTA	894.5
chr14.trna19-TyrGTA	364.5	chr14.trna19-TyrGTA	884.5
chr6.trna124-ArgTCG	237.5	chr7.trna7-CysGCA	681.5
chr6.trna14-TyrGTA	223.5	chr1.trna105-GlnCTG	591.0
chr12.trna12-AspGTC	214.0	chr6.trna52-ArgTCT	581.0
chr6.trna145-SerAGA	211.0	chr6.trna124-ArgTCG	565.5
chr6.trna52-ArgTCT	200.5	chr16.trna1-ArgCCG	544.5

(b)  

mRNA	Accession	Serum	mRNA	Accession	Exosomes
CCDC96	NM_153376	251.5	CCDC96	NM_153376	606.5
SIRPG	NM_080816	240.5	HGSNAT	NM_152419	503.0
TBX22	NM_016954	215.0	TBX22	NM_016954	465.0
RNF223	NM_001205252	200.5	SIRPG	NM_018556	350.0
SIRPG	NM_018556	198.0	SIRPG	NM_080816	340.0
NFIX	NM_002501	198.0	NFIX	NM_002501	338.5
RAP2A	NM_021033	174.0	GPR126	NM_198569	288.0
SIRPG	NM_001039508	154.0	ILDR2	NM_199351	273.5
HGSNAT	NM_152419	143.0	PAPOLB	NM_020144	267.5
RBFOX1	NM_001142333	129.5	SIRPG	NM_001039508	246.5
ncRNA	Accession	Serum	ncRNA	Accession	Exosomes
RNY4	NR_004393	1043.5	RNY4	NR_004393	704.5
RNY5	NR_001571	477.0	RNY5	NR_001571	262.0
RNY3	NR_004392	360.0	RNY3	NR_004392	174.0
RNY4P8	NR_033356	177.0	FLJ33534	NR_040080	167.0
RNU2-1	NR_002716	159.5	LOC100130673	NR_038454	155.0
RNY1	NR_004391	145.5	LOC100507605	NR_038375	153.0
RNU12	NR_029422	127.5	C20orf173	NR_026933	152.0
C1orf213	NR_033690	101.0	LOC100507605	NR_038374	139.5
RNU5B-1	NR_002757	71.0	LOC644936	NR_004845	136.0
C20orf173	NR_026933	70.5	LOC100507605	NR_038376	130.5