Harmine and Harmaline Downregulate TCDD-Induced Cyp1a1 in the Livers and Lungs of C57BL/6 Mice
Figure 4
Effect of harmine on TCDD-mediated induction of lung Cyp1a1 at mRNA (a), protein (b), and catalytic activity (c) in C57BL/6 mice. Mice were distributed into several groups, receiving the used vehicle (weight-matched control), TCDD, or TCDD and harmine. After 14 h from TCDD treatment, mice were sacrificed, and the lungs were isolated. Total RNA was isolated using TRIzol reagent, and microsomal fractions were isolated using ultracentrifugation. The level of Cyp1a1 mRNA was determined using real-time PCR (a). Furthermore, Cyp1a protein and Cyp1a1 catalytic activity were determined in microsomal fractions using Western blot analysis (b) and 7ER as a substrate (c), respectively. Values represent the mean ± S.E.M. (). (+) compared to control (c), (*) compared to .
