Table 3: Effect of benzene fraction of Murraya koenigii on the mutagenicity induced by methyl methanesulfonate (MMS) using Salmonella typhimurium strains.

Treatment
Dose (µg/plate)Number of His+ revertants colonies/plate
TA97aTA98TA100TA102

Spontaneous140.0 ± 4.031.0 ± 3.6128.7 ± 9.8245.7 ± 23.3
Positive control (MMS)1443.7 ± 19.951.0 ± 2.7952.7 ± 46.01195.3 ± 79.0
Murraya koenigii 12.5164.3 ± 12.346.0 ± 4.0191.7 ± 13.3286.7 ± 26.3
25145.0 ± 15.537.3 ± 7.0172.3 ± 18.9260.0 ± 24.1
50124.7 ± 9.130.7 ± 8.5153.0 ± 19.7245.3 ± 24.4
100118.0 ± 10.524.0 ± 4.6127.0 ± 15.5228.3 ± 19.6
  koenigii+ MMS
12.5388.3 ± 31.2 (19.8)50.3 ± 4.0 (13.4)802.3 ± 57.1* (19.8)862.3 ± 58.8** (36.6)
25309.0 ± 30.8* (45.1)45.0 ± 4.6 (43.9)565.0 ± 45.1*** (49.7)675.3 ± 56.9*** (55.6)
50239.7 ± 20.2** (63.9)38.3 ± 4.0* (62.3)440.7 ± 48.6*** (64.0)518.0 ± 32.1*** (71.3)
100183.0 ± 17.5*** (80.0)31.0 ± 5.6** (74.1)262.3 ± 31.7*** (83.6)363.7 ± 37.1*** (86.0)

  value treatment81.6359.877225.881178.255

  value0.9890.9580.9780.996

The data represented in the table is the mean ± SD values of three replicates. Mean values followed by asterisk are significantly different when compared to positive control at *P < 0.05; **P < 0.005 and ***P < 0.001. The values in parentheses are the inhibition rates of mutagenicity (%). Positive control: MMS: methyl methanesulfonate. aNegative control; bPre-incubation; : Linear regression analysis.