Table 5: Effect of benzene fraction of Murraya koenigii on the mutagenicity induced by 2-aminofluorene (2-AF) with metabolic activation using Salmonella typhimurium strains.

TreatmentDose (µg/plate)Number of His+ revertants colonies/plate
TA97aTA98TA100TA102

Spontaneous143.7 ± 13.237.0 ± 3.0135.3 ± 9.5316.0 ± 14.5
Positive control (2AF)5328.0 ± 12.0262.3 ± 17.2502.0 ± 19.11430.3 ± 32.5
Murraya koenigii 12.5142.0 ± 18.350.3 ± 2.5132.7 ± 16.0265.0 ± 28.2
25156.0 ± 14.442.7 ± 3.5155.3 ± 17.2296.3 ± 17.6
50168.7 ± 21.735.0 ± 3.6 322.7 ± 31.1
100181.3 ± 27.128.0 ± 3.6175.3 ± 20.6340.3 ± 31.3
Murraya koenigii+ 2-AF12.5276.3 ± 31.1 (27.8)200.7 ± 17.0* (29.1)422.3 ± 32.7* (21.6)1185.3 ± 77.0** (21.0)
25248.7 ± 28.0* (46.1)161.0 ± 12.5** (46.1)346.3 ± 21.6*** (44.9)960.7 ± 70.8*** (41.4)
50220.0 ± 25.0** (67.8)114.7 ± 13.1*** (65.0)280.7 ± 27.0*** (66.3)655.0 ± 55.3*** (70.0)
100202.3 ± 14.2*** (85.7)70.0 ± 7.6*** (82.1)205.0 ± 18.0*** (90.9)462.0 ± 32.9*** (88.8)

value treatment24.643197.23937.820276.179

value0.9870.9990.9990.994

The data represented in the table is the mean ± SD values of three replicates. Mean values followed by asterisk are significantly different when compared to positive control at *P < 0.05; **P < 0.005 and ***P < 0.001. The values in parentheses are the inhibition rates of mutagenicity (%). Positive control: 2-AF, 2-aminoflourene.aNegative control; bPre-incubation test; : Linear regression analysis.