Development of a Broad-Range 23S rDNA Real-Time PCR Assay for the Detection and Quantification of Pathogenic Bacteria in Human Whole Blood and Plasma Specimens

Figure 2

Standard curve amplification of cloned bacterial 23S rDNA plasmid real-time PCR ranging from 10⁸ to 10¹ copies per reaction. Panel (a) shows the amplification curve constructed by PCR assay. The fluorescence and the corresponding cycle numbers are shown in the vertical and horizontal axes, respectively. Panel (b) shows the relative standard curve ranging from 10¹ to 10⁸ copies per reaction. Panel (c) shows the corresponding melting curve.

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