264651.fig.002a
(a)
264651.fig.002b
(b)
264651.fig.002c
(c)
Figure 2: Standard curve amplification of cloned bacterial 23S rDNA plasmid real-time PCR ranging from 108 to 101 copies per reaction. Panel (a) shows the amplification curve constructed by PCR assay. The fluorescence and the corresponding cycle numbers are shown in the vertical and horizontal axes, respectively. Panel (b) shows the relative standard curve ranging from 101 to 108 copies per reaction. Panel (c) shows the corresponding melting curve.