Development of a Broad-Range 23S rDNA Real-Time PCR Assay for the Detection and Quantification of Pathogenic Bacteria in Human Whole Blood and Plasma Specimens
Figure 4
Analytical detection range and sensitivities of different extraction protocols from whole blood (continuous line) spiked with Gram-negative (E. coli) or Gram-positive (S. aureus) bacteria and their corresponding plasma (dotted line). EasyMag protocols with E. coli (a) or with S. aureus (d), spiked in plasma and in whole blood. QIAmp DNA blood mini kit extraction with E. coli (b) or with S. aureus (e), spiked in plasma and in whole blood; QIAmp DNA blood mini kit extraction with pre-treatment with lysozime and proteinase K with E. coli (c) or with S. aureus (f), in plasma and in whole blood.
