Research Article

An Immunohistochemistry Study of Sox9, Runx2, and Osterix Expression in the Mandibular Cartilages of Newborn Mouse

Figure 1

Histological analysis of mandibular cartilages of mice at newborn stage. (a) Lingual view of mandible by Alizarin Red and Alcian Blue staining; (b) hematoxylin-eosin sections of condylar cartilage, and (c) hematoxylin-eosin sections of angular cartilage (AG) similarly displaying four different cell zones: a thin fibrous cell zone (F), a polymorphic cell zone (P), a wider flattened cell zone (FL), and a broad hypertrophic cell zone (HY); (d) hematoxylin-eosin staining pattern of the matrix of the posterior portion of intramandibular Meckel’s cartilage (PM) changed from the intense hematoxylin to the light eosin in the resorption (R) facing the molar buds (Mo) and incisor (I); (e) hematoxylin-eosin sections of the endochondral ossification rostral process of Meckel’s cartilage (RC) and symphyseal secondary cartilage (SS) facing the incisor (I). Scale bar: 100 μm for (a) and (b), 250 μm for (c) and (d), and 50 μm for (e).
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