Constitutive Optimized Production of Streptokinase in Saccharomyces cerevisiae Utilizing Glyceraldehyde 3-Phosphate Dehydrogenase Promoter of Pichia pastoris

Table 1

Stability of intracellularly expressed SK in S. cerevisiae cell lysates.

Storage temperature^a	% Functional activity^b in Cell lysis supernatant
−20°C	100
0°C	94
4°C	86
25°C	9
37°C	—
42°C	—

aCell lysis supernatant of Saccharomyces clone expressing SK was stored at above temperatures for duration of 96 hrs.
bFunctional activity was assayed by in vitro clot lysis method and is a mean of three assays.