Total 25-Hydroxyvitamin D Determination by an Entry Level Triple Quadrupole Instrument: Comparison between Two Commercial Kits

Table 1

Compounds dependent MS/MS parameters for Chromsystems (a) and for PerkinElmer (b) assays.

(a)

Chromsystems
Compound	SRM	Collision Energy [eV]	Tube Lens [V]
25(OH)D3	383.2 → 210.8	23	80
25(OH)D2	395.2 → 268.8	17	80
25(OH)D3/D2 IS	389.3 → 210.8	28	80

(b)

PerkinElmer
Compound	SRM	Collision Energy [eV]	Tube Lens [V]
25(OH)D3 Cal	613.4 → 298.1	19	110
25(OH)D3 IS	610.1 → 310.1	19	110
25(OH)D3	607.4 → 298.1	19	110
25(OH)D2 Cal	625.4 → 298.1	17	110
25(OH)D2 IS	622.4 → 301.1	17	110
25(OH)D2	619.4 → 298.1	17	110