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Figure 5: NSOM/QD-based dual-color imaging of CD3 and GM1 for T-cells costimulated with anti-CD3/anti-CD28 Ab after PKCθ inhibitor Rottlerin pretreatment. (a) T-cell topography, (b) fluorescence image of QD-bound CD3 (red). (c) Fluorescence image of QD-bound GM1 (blue). (d) Two color fluorescence merge image. (e) Topography-fluorescence merge image. ((f), (g), and (h)) Zoom images of the areas as indicated by the squares on (b), (c) and (d), respectively. (i) The percentage numbers of TCR/CD3 or GM1 molecules that arrayed to form nanodomains. (j) Molecule density of TCR/CD3 nanodomains or GM1 nanodomains. Scale bars are 1 μm for (a), (b), (c), (d) and (e) and 200 nm for (f), (g) and (h).