Optimisation of the Enzymatic Hydrolysis of Blood Cells with a Neutral Protease

Figure 3

Contour plots for the effect of variables on the DH as a function of various hydrolysis conditions: (a) pH and temperature, (b) pH and E/S, (c) pH and incubation period, (d) temperature and E/S, (e) temperature and incubation period, (f) E/S and incubation period.

(a) Fixed levels: E/S = 0.10, Incubation period = 10.00 h

(b) Fixed levels: Temperature = 50.00°C, Incubation period = 10.00 h

(c) Fixed levels: Temperature = 50.00°C, E/S = 0.10

(d) Fixed levels: pH = 7.00, Incubation period = 10.00 h

(e) Fixed levels: pH = 7.00, E/S = 0.10

(f) Fixed levels: pH = 7.00, Temperature = 50.00°C