Optimisation of the Enzymatic Hydrolysis of Blood Cells with a Neutral Protease
Table 2
The degree of hydrolysis at various pH values, temperatures, E/S values, and incubation periods.
Run
Code values
Real values
DH*
Experimental
Predicted
1
0
0
0
0
7.00
50.00
0.10
10.00
28.60
2
−1
−1
1
−1
6.50
45.00
0.11
8.00
30.97
3
−1
1
1
−1
6.50
55.00
0.11
8.00
26.25
4
−1
1
1
1
6.50
55.00
0.11
12.00
31.73
5
1
1
−1
−1
7.50
55.00
0.08
8.00
25.49
6
0
0
0
2
7.00
50.00
0.10
14.00
36.10
7
2
0
0
0
8.00
50.00
0.10
10.00
30.21
8
1
−1
1
1
7.50
45.00
0.11
12.00
35.01
9
−1
−1
−1
1
6.50
45.00
0.08
12.00
29.85
10
0
0
0
0
7.00
50.00
0.10
10.00
28.60
11
1
1
1
1
7.50
55.00
0.11
12.00
32.24
12
1
−1
−1
−1
7.50
45.00
0.08
8.00
28.71
13
0
0
0
0
7.00
50.00
0.10
10.00
28.60
14
0
0
0
0
7.00
50.00
0.10
10.00
28.60
15
0
0
0
0
7.00
50.00
0.10
10.00
28.60
16
0
0
−2
0
7.00
50.00
0.06
10.00
23.25
17
−1
1
−1
1
6.50
55.00
0.08
12.00
27.32
18
0
0
2
0
7.00
50.00
0.13
10.00
28.77
19
1
1
−1
1
7.50
55.00
0.08
12.00
29.31
20
0
2
0
0
7.00
60.00
0.10
10.00
26.69
21
−1
−1
1
1
6.50
45.00
0.11
12.00
35.13
22
−1
−1
−1
−1
6.50
45.00
0.08
8.00
28.39
23
1
−1
−1
1
7.50
45.00
0.08
12.00
31.21
24
0
0
0
0
7.00
50.00
0.10
10.00
28.60
25
1
1
1
−1
7.50
55.00
0.11
8.00
25.73
26
0
−2
0
0
7.00
40.00
0.10
10.00
33.31
27
1
−1
1
−1
7.50
45.00
0.11
8.00
29.82
28
−2
0
0
0
6.00
50.00
0.10
10.00
29.38
29
0
0
0
−2
7.00
50.00
0.10
6.00
28.13
30
−1
1
−1
−1
6.50
55.00
0.08
8.00
24.54
*DH represents the average degree of hydrolysis of triplicate experiments. The DH was calculated using the equation () derived from the standard curve of completely hydrolyzed BCs (absorbance at 570 nm versus the concentration of the hydrolyzate).
a: pH.
b: temperature.
c: E/S.
d: incubation period.
