Table 2: The degree of hydrolysis at various pH values, temperatures, E/S values, and incubation periods.

RunCode valuesReal valuesDH*
ExperimentalPredicted

1 0 0 0 0 7.0050.000.1010.00 28.60
2−1−1 1 −16.5045.000.118.00 30.97
3−1 1 1 −16.5055.000.118.00 26.25
4−1 1 1 1 6.5055.000.1112.00 31.73
5 1 1 −1−17.5055.000.088.00 25.49
6 0 0 0 2 7.0050.000.1014.00 36.10
7 2 0 0 0 8.0050.000.1010.00 30.21
8 1 −1 1 1 7.5045.000.1112.00 35.01
9−1−1−1 1 6.5045.000.0812.00 29.85
10 0 0 0 0 7.0050.000.1010.00 28.60
11 1 1 1 1 7.5055.000.1112.00 32.24
12 1 −1−1−17.5045.000.088.00 28.71
13 0 0 0 0 7.0050.000.1010.00 28.60
14 0 0 0 0 7.0050.000.1010.00 28.60
15 0 0 0 0 7.0050.000.1010.00 28.60
16 0 0 −2 0 7.0050.000.0610.00 23.25
17−1 1 −1 1 6.5055.000.0812.00 27.32
18 0 0 2 0 7.0050.000.1310.00 28.77
19 1 1 −1 1 7.5055.000.0812.00 29.31
20 0 2 0 0 7.0060.000.1010.00 26.69
21−1−1 1 1 6.5045.000.1112.00 35.13
22−1−1−1−16.5045.000.088.00 28.39
23 1 −1−1 1 7.5045.000.0812.00 31.21
24 0 0 0 0 7.0050.000.1010.00 28.60
25 1 1 1 −17.5055.000.118.00 25.73
26 0 −2 0 0 7.0040.000.1010.00 33.31
27 1 −1 1 −17.5045.000.118.00 29.82
28−2 0 0 0 6.0050.000.1010.00 29.38
29 0 0 0 −27.0050.000.106.00 28.13
30−1 1 −1−16.5055.000.088.00 24.54

*DH represents the average degree of hydrolysis of triplicate experiments. The DH was calculated using the equation ( ) derived from the standard curve of completely hydrolyzed BCs (absorbance at 570 nm versus the concentration of the hydrolyzate).
a : pH.
b : temperature.
c : E/S.
d : incubation period.