Optimisation of the Enzymatic Hydrolysis of Blood Cells with a Neutral Protease

Table 3

ANOVA for the response surface quadratic polynomial model.

Source	Sum of squares	df	Mean square	𝐹 value	𝑃 value	Significance
Model	0.03	14	1.88𝐸−03	20.16	<0.00	**^a
𝑋1—pH	1.04𝐸−04	1	1.04𝐸−04	1.11	0.39
𝑋2—temperature	6.58𝐸−03	1	6.58𝐸−03	70.40	<0.00	**
𝑋3—E/S	4.56𝐸−03	1	4.56𝐸−03	48.84	<0.00	**
𝑋4—incubation period	9.53𝐸−03	1	9.53𝐸−03	102.04	<0.00	**
𝑋1𝑋2	4.00𝐸−05	1	4.00𝐸−05	0.43	0.52
𝑋1𝑋3	2.18𝐸−04	1	2.18𝐸−04	2.34	0.15
𝑋1𝑋4	1.08𝐸−04	1	1.08𝐸−04	1.15	0.30
𝑋2𝑋3	7.53𝐸−05	1	7.53𝐸−05	0.81	0.38
𝑋2𝑋4	1.74𝐸−04	1	1.74𝐸−04	1.86	0.19
𝑋3𝑋4	7.28𝐸−04	1	7.28𝐸−04	7.79	0.01	****^c
𝑋^21	2.44𝐸−04	1	2.44𝐸−04	2.62	0.13
𝑋^22	3.38𝐸−04	1	3.38𝐸−04	3.62	0.08
𝑋^23	1.15𝐸−03	1	1.15𝐸−03	12.32	0.00	***^b
𝑋^24	2.12𝐸−03	1	2.12𝐸−03	22.72	0.00	**
Residual	1.40𝐸−03	15	9.34𝐸−05
Lack of fit	6.45𝐸−04	10	6.45𝐸−05	0.43	0.88
Pure error	7.57𝐸−04	5	1.51𝐸−04
Cor. total	0.03	29
Std. Dev.	9.67𝐸−03			𝑅-squared	0.95
Mean	0.29			Adj. 𝑅-squared	0.90
C.V.%	3.30			Pred. 𝑅-squared	0.83
PRESS	4.80𝐸−03			Adeq. precisior	18.81

df: degree of freedom.
^aSignificance at 0.001 level.
^bSignificance at 0.01 level.
^cSignificance at 0.05 level