Secretion of Biologically Active Heterologous Oxalate Decarboxylase (OxdC) in Lactobacillus plantarum WCFS1 Using Homologous Signal Peptides

Figure 2

Silver stained gels after SDS-PAGE of supernatant of lactobacillus strain harboring various plasmids. All cells were induced with 50 ng inducing peptide mL⁻¹ at OD₆₀₀ ~ 0.3 and harvested at OD₆₀₀ ~ 1.7; lane M, molecular weight marker (kDa); lane 1, strain harboring pSIP-OxdC; lane 2, pLp_3050sOxdC; lane 3 pLp_0373sOxdC; arrow indicate, the location of OxdC protein (calculated molecular mass of 44 kDa).