About this Journal Submit a Manuscript Table of Contents
BioMed Research International
Volume 2013 (2013), Article ID 280432, 9 pages
http://dx.doi.org/10.1155/2013/280432
Research Article

Secretion of Biologically Active Heterologous Oxalate Decarboxylase (OxdC) in Lactobacillus plantarum WCFS1 Using Homologous Signal Peptides

1Department of Biochemistry, Centre for Advanced Studies in Organismal and Functional Genomics, School of Biological Sciences, Madurai Kamaraj University, Madurai 625 021, India
2INSERM-U844, Institut des Neuroscience de Montpellier Building, Hopital St. Eloi, 34091 Montpellier, France

Received 22 April 2013; Accepted 28 June 2013

Academic Editor: Isabel Sá-Correia

Copyright © 2013 Ponnusamy Sasikumar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. E. Leumann and B. Hoppe, “The primary hyperoxalurias,” Journal of the American Society of Nephrology, vol. 12, no. 9, pp. 1986–1993, 2001. View at Scopus
  2. E. Hylander, S. Jarnum, H. J. Jensen, and M. Thale, “Enteric hyperoxaluria: dependence on small intestinal resection, colectomy, and steatorrhoea in chronic inflammatory bowel disease,” Scandinavian Journal of Gastroenterology, vol. 13, no. 5, pp. 577–588, 1978. View at Scopus
  3. J. H. Clark, J. F. Fitzgerald, and J. M. Bergstein, “Nephrolithiasis in childhood inflammatory bowel disease,” Journal of Pediatric Gastroenterology and Nutrition, vol. 4, no. 5, pp. 829–834, 1985. View at Scopus
  4. L. A. Matthews, C. F. Doershuk, R. C. Stern, and M. I. Resnick, “Urolithiasis and cystic fibrosis,” The Journal of Urology, vol. 155, no. 5, pp. 1563–1564, 1996. View at Publisher · View at Google Scholar · View at Scopus
  5. R. Kumar, M. Mukherjee, M. Bhandari, A. Kumar, H. Sidhu, and R. D. Mittal, “Role of Oxalobacter formigenes in calcium oxalate stone disease: a study from North India,” European Urology, vol. 41, no. 3, pp. 318–322, 2002. View at Publisher · View at Google Scholar · View at Scopus
  6. B. Hoppe, E. Leumann, G. von Unruh, N. Laube, and A. Hesse, “Diagnostic and therapeutic approaches in patients with secondary hyperoxaluria,” Frontiers in Bioscience, vol. 8, pp. 437–443, 2003. View at Scopus
  7. H. Sidhu, M. J. Allison, J. M. Chow, A. Clark, and A. B. Peck, “Rapid reversal of hyperoxaluria in a rat model after probiotic administration of Oxalobacter formigenes,” The Journal of Urology, vol. 166, no. 4, pp. 1487–1491, 2001. View at Scopus
  8. S. Hazebrouck, R. Oozeer, K. Adel-Patient et al., “Constitutive delivery of bovine β-lactoglobulin to the digestive tracts of gnotobiotic mice by engineered Lactobacillus casei,” Applied and Environmental Microbiology, vol. 72, no. 12, pp. 7460–7467, 2006. View at Publisher · View at Google Scholar · View at Scopus
  9. B. Hoppe, J. W. Groothoff, S.-A. Hulton et al., “Efficacy and safety of Oxalobacter formigenes to reduce urinary oxalate in primary hyperoxaluria,” Nephrology Dialysis Transplantation, vol. 26, no. 11, pp. 3609–3615, 2011. View at Publisher · View at Google Scholar · View at Scopus
  10. A. Ljungh and T. Wadstrom, Lactobacillus Molecular Biology: From Genomics to Probiotics, Caister Academic Press, Norfolk, UK, 2009.
  11. J. M. Wells and A. Mercenier, “Mucosal delivery of therapeutic and prophylactic molecules using lactic acid bacteria,” Nature Reviews Microbiology, vol. 6, no. 5, pp. 349–362, 2008. View at Publisher · View at Google Scholar · View at Scopus
  12. B. Del Rio, R. J. Dattwyler, M. Aroso et al., “Oral immunization with recombinant Lactobacillus plantarum induces a protective immune response in mice with lyme disease,” Clinical and Vaccine Immunology, vol. 15, no. 9, pp. 1429–1435, 2008. View at Publisher · View at Google Scholar · View at Scopus
  13. P. Marteau, P. Seksik, and R. Jian, “Probiotics and intestinal health effects: a clinical perspective,” British Journal of Nutrition, vol. 88, no. 1, pp. S51–S57, 2002. View at Scopus
  14. S. Prakash and C. Martoni, “Toward a new generation of therapeutics: artificial cell targeted delivery of live cells for therapy,” Applied Biochemistry and Biotechnology, vol. 128, no. 1, pp. 1–21, 2006. View at Scopus
  15. L. Steidler, W. Hans, L. Schotte et al., “Treatment of murine colitis by Lactococcus lactis secreting interleukin-10,” Science, vol. 289, no. 5483, pp. 1352–1355, 2000. View at Publisher · View at Google Scholar · View at Scopus
  16. L. Steidler, S. Neirynck, N. Huyghebaert et al., “Biological containment of genetically modified Lactococcus lactis for intestinal delivery of human interleukin 10,” Nature Biotechnology, vol. 21, no. 7, pp. 785–789, 2003. View at Publisher · View at Google Scholar · View at Scopus
  17. S. Hazebrouck, R. Oozeer, K. Adel-Patient et al., “Constitutive delivery of bovine β-lactoglobulin to the digestive tracts of gnotobiotic mice by engineered Lactobacillus casei,” Applied and Environmental Microbiology, vol. 72, no. 12, pp. 7460–7467, 2006. View at Publisher · View at Google Scholar · View at Scopus
  18. C. Krüger, Y. Hu, Q. Pan, et al., “In situ delivery of passive immunity by Lactobacillus producing single-chain antibodies,” Nature Biotechnology, vol. 20, no. 7, pp. 702–706, 2002. View at Publisher · View at Google Scholar
  19. J. C. Lieske, W. J. Tremaine, C. De Simone et al., “Diet, but not oral probiotics, effectively reduces urinary oxalate excretion and calcium oxalate supersaturation,” Kidney International, vol. 78, no. 11, pp. 1178–1185, 2010. View at Publisher · View at Google Scholar · View at Scopus
  20. B. C. Jeong, D. H. Han, S. I. Seo, et al., “Yvrk gene recombinant E. coli reduce the concentration of urine oxalate in transient Hyperoxaluria rat model,” The Journal of Urology, vol. 181, p. 660, 2009.
  21. D. Grujic, E. C. Salido, B. C. Shenoy et al., “Hyperoxaluria is reduced and nephrocalcinosis prevented with an oxalate-degrading enzyme in mice with hyperoxaluria,” American Journal of Nephrology, vol. 29, no. 2, pp. 86–93, 2009. View at Publisher · View at Google Scholar · View at Scopus
  22. A. B. Cowley, D. W. Poage, R. R. Dean et al., “14-Day repeat-dose oral toxicity evaluation of oxazyme in rats and dogs,” International Journal of Toxicology, vol. 29, no. 1, pp. 20–31, 2010. View at Publisher · View at Google Scholar · View at Scopus
  23. A. Kolandaswamy, L. George, and S. Sadasivam, “Heterologous expression of oxalate decarboxylase in Lactobacillus plantarum NC8,” Current Microbiology, vol. 58, no. 2, pp. 117–121, 2009. View at Publisher · View at Google Scholar · View at Scopus
  24. G. Mathiesen, A. Sveen, M. B. Brurberg, L. Fredriksen, L. Axelsson, and V. G. H. Eijsink, “Genome-wide analysis of signal peptide functionality in Lactobacillus plantarum WCFS1,” BMC Genomics, vol. 10, article 1471, p. 425, 2009. View at Publisher · View at Google Scholar · View at Scopus
  25. M. Kleerebezem, J. Boekhorst, R. Van Kranenburg et al., “Complete genome sequence of Lactobacillus plantarum WCFS1,” Proceedings of the National Academy of Sciences of the United States of America, vol. 100, no. 4, pp. 1990–1995, 2003. View at Publisher · View at Google Scholar · View at Scopus
  26. V. J. Just, C. E. M. Stevenson, L. Bowater, A. Tanner, D. M. Lawson, and S. Bornemann, “A closed conformation of Bacillus subtilis oxalate decarboxylase OxdC provides evidence for the true identity of the active site,” The Journal of Biological Chemistry, vol. 279, no. 19, pp. 19867–19874, 2004. View at Publisher · View at Google Scholar · View at Scopus
  27. J. Sambrook and D. Russel, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, New York, NY, USA, 2001.
  28. T. W. Aukrust, M. B. Brurberg, and I. F. Nes, “Transformation of Lactobacillus by electroporation,” Methods in Molecular Biology, vol. 47, pp. 201–208, 1995. View at Scopus
  29. M. B. Brurberg, I. F. Nes, and V. G. H. Eijsink, “Pheromone-induced production of antimicrobial peptides in Lactobacillus,” Molecular Microbiology, vol. 26, no. 2, pp. 347–360, 1997. View at Scopus
  30. E. Sørvig, S. Grönqvist, K. Naterstad, G. Mathiesen, V. G. H. Eijsink, and L. Axelsson, “Construction of vectors for inducible gene expression in Lactobacillus sakei and L. plantarum,” FEMS Microbiology Letters, vol. 229, no. 1, pp. 119–126, 2003. View at Publisher · View at Google Scholar · View at Scopus
  31. G. Mathiesen, A. Sveen, J.-C. Piard, L. Axelsson, and V. G. H. Eijsink, “Heterologous protein secretion by Lactobacillus plantarum using homologous signal peptides,” Journal of Applied Microbiology, vol. 105, no. 1, pp. 215–226, 2008. View at Publisher · View at Google Scholar · View at Scopus
  32. U. K. Laemmli, “Cleavage of structural proteins during the assembly of the head of bacteriophage T4,” Nature, vol. 227, no. 5259, pp. 680–685, 1970. View at Publisher · View at Google Scholar · View at Scopus
  33. G. Mathiesen, E. Sørvig, J. Blatny, K. Naterstad, L. Axelsson, and V. G. H. Eijsink, “High-level gene expression in Lactobacillus plantarum using a pheromone-regulated bacteriocin promoter,” Letters in Applied Microbiology, vol. 39, no. 2, pp. 137–143, 2004. View at Publisher · View at Google Scholar · View at Scopus
  34. A. Tanner and S. Bornemann, “Bacillus subtilis yvrK is an acid-induced oxalate decarboxylase,” Journal of Bacteriology, vol. 182, no. 18, pp. 5271–5273, 2000. View at Publisher · View at Google Scholar · View at Scopus
  35. L. Axelsson and S. Ahrne, “Lactic acid bacteria,” in Applied Microbial Systematics, F. G. Priest and M. Goodfellow, Eds., pp. 365–386, Kluwer Academic Publishers, Dordrecht, The Netherlands, 2000.
  36. G. Perez-Martinez, J. Kok, G. Venema, J. M. Van Dijl, H. Smith, and S. Bron, “Protein export elements from Lactococcus lactis,” Molecular and General Genetics, vol. 234, no. 3, pp. 401–411, 1992. View at Publisher · View at Google Scholar · View at Scopus
  37. A. Bolhuis, H. Tjalsma, H. E. Smith et al., “Evaluation of bottlenecks in the late stages of protein secretion in Bacillus subtilis,” Applied and Environmental Microbiology, vol. 65, no. 7, pp. 2934–2941, 1999. View at Scopus
  38. U. Brockmeier, M. Caspers, R. Freudl, A. Jockwer, T. Noll, and T. Eggert, “Systematic screening of all signal peptides from Bacillus subtilis: a powerful strategy in optimizing heterologous protein secretion in Gram-positive bacteria,” Journal of Molecular Biology, vol. 362, no. 3, pp. 393–402, 2006. View at Publisher · View at Google Scholar · View at Scopus
  39. S. Turroni, C. Bendazzoli, S. C. F. Dipalo et al., “Oxalate-degrading activity in Bifidobacterium animalis subsp. lactis: impact of acidic conditions on the transcriptional levels of the oxalyl coenzyme A (CoA) decarboxylase and formyl-CoA ransferase genes,” Applied and Environmental Microbiology, vol. 76, no. 16, pp. 5609–5620, 2010. View at Publisher · View at Google Scholar · View at Scopus
  40. E. Sørvig, G. Mathiesen, K. Naterstad, V. G. H. Eijsink, and L. Axelsson, “High-level, inducible gene expression in Lactobacillus sakei and Lactobacillus plantarum using versatile expression vectors,” Microbiology, vol. 151, no. 7, pp. 2439–2449, 2005. View at Publisher · View at Google Scholar · View at Scopus
  41. E. Halbmayr, G. Mathiesen, T.-H. Nguyen et al., “High-level expression of recombinant β-galactosidases in Lactobacillus plantarum and Lactobacillus sakei using a sakacin p-based expression system,” Journal of Agricultural and Food Chemistry, vol. 56, no. 12, pp. 4710–4719, 2008. View at Publisher · View at Google Scholar · View at Scopus
  42. Y. Le Loir, A. Gruss, S. D. Ehrlich, and P. Langella, “A nine-residue synthetic propeptide enhances secretion efficiency of heterologous proteins in Lactococcus lactis,” Journal of Bacteriology, vol. 180, no. 7, pp. 1895–1903, 1998. View at Scopus
  43. P. W. Mufarrij, J. N. Lange, J. Knight, et al., “Second place: the effects of oxazyme on oxalate degradation: results and implications of In Vitro experiments,” Journal of Endourology, vol. 27, no. 3, pp. 284–287, 2013. View at Publisher · View at Google Scholar
  44. T.-T. Nguyen, G. Mathiesen, L. Fredriksen et al., “A food-grade system for inducible gene expression in Lactobacillus plantarum using an alanine racemase-encoding selection marker,” Journal of Agricultural and Food Chemistry, vol. 59, no. 10, pp. 5617–5624, 2011. View at Publisher · View at Google Scholar · View at Scopus