Table 1: Bacterial strains and plasmids used in this study.

Strains and plasmidsCharacteristicsaSource/references

Strains
 E. coli DH10B Host strain for cloning commercialInvitrogen
 L. plantarum WCFS1*Host strain, plasmid-free, silage isolateKleerebezem et al. (2003) [25]
Plasmid
pLp_0373sAmyAp256/pUC(pGEM)ori; PsppA; Lp_0373; amyA; EmrMathiesen et al. (2009) [24]
pLp_3050sAmyAp256/pUC(pGEM)ori; PsppA; Lp_3050; amyA; EmrMathiesen et al. (2009) [24]
pLB36pET 32a oxdC; AmrJust et al. (2004) [26]
pSip-OxdC p256/pUC(pGEM)ori; PsppA; oxdC; EmrKolandaswamy et al. (2009) [23]
pLp_0373sOxdCp256/pUC(pGEM)ori; PsppA; spLp_0373 fused to the oxdC; EmrThis work
pLp_3050sOxdCp256/pUC(pGEM)ori; PsppA; spLp_3050fused to the oxdC; EmrThis work

For strains, genotypic and phenotypic characteristics are given; for plasmid, plasmid and cloned-cassette characteristics are given; Emr, Amr: resistance to erythromycin and ampicillin, respectively.
L. plantarum WCFS1 is a single colony isolate of strain NCIM8826 (Kleerebezem et al., 2003 [25]).