Expression and Complex Formation of MMP9, MMP2, NGAL, and TIMP1 in Porcine Myocardium but Not in Skeletal Muscles in Male Pigs with Tachycardia-Induced Systolic Heart Failure
Figure 2
Representative Western blots for MMP9, MMP2, NGAL and TIMP1 detection in porcine homogenates of LV myocardium (a) and BF (b) in nonreducing and nondenaturing conditions. Positive controls in nonreducing and nondenaturing conditions (c). Representative coimmunoprecipitates of porcine lysates of LV myocardium immunoprecipited with anti-MMP9 antibody, and subsequently blotted with anti-MMP2, anti-NGAL, and anti-TIMP1 antibodies (d). Representative semiquantitative Western blots of porcine homogenates of LV myocardium using anti-MMP9, anti-TIMP1, and anti-NGAL antibodies (e).
