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BioMed Research International
Volume 2013 (2013), Article ID 283964, 6 pages
http://dx.doi.org/10.1155/2013/283964
Research Article

Characterization of an Antihypertensive Angiotensin I-Converting Enzyme Inhibitory Peptide from the Edible Mushroom Hypsizygus marmoreus

1Department of Biomedicinal Science and Biotechnology, PaiChai University, Daejeon 302-735, Republic of Korea
2Korea National College of Agricultural and Fishery, Hwasung, Kyonggi-do 445-893, Republic of Korea

Received 14 June 2013; Accepted 4 September 2013

Academic Editor: Youngjun Choi

Copyright © 2013 Min-Gu Kang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Hypertension is one of the very serious diseases and, recently, hypertensive patient longevity has been increased significantly. Therefore, the development of new antihypertensive drugs or bioactive compounds is very important to remedy or prevent hypertension. The antihypertensive angiotensin I-converting enzyme (ACE) inhibitor in water extracts from the brown-cultivar-fruiting-body of Hypsizygus marmoreus was purified with ultrafiltration, C18 solid phase extraction chromatography and reverse-phase HPLC, and the purified ACE inhibitor with inhibitory activity of IC50 value of 0.19 mg/mL was obtained. The purified ACE inhibitor was found to be a new oligopeptide with the sequence LSMGSASLSP. Its molecular weight was estimated to be 567.3 Da and the water extracts containing ACE inhibitor from Hypsizygus marmoreus showed a clear antihypertensive action a spontaneously hypertensive rat.