Analysis of Activated Platelet-Derived Growth Factor β Receptor and Ras-MAP Kinase Pathway in Equine Sarcoid Fibroblasts

Figure 7

Dose-dependent inhibition of PDGF-BB-induced PDFGβR activation by imatinib mesylate. In sarcoid-derived cell lines (EqS) treated with increasing doses of imatinib with or without PDGF-BB, the drug inhibited PDFGβR autophosphorylation. PDGFβR downregulation was another measure of its activation induced by PDFG-BB, and, conversely, increasing of PDGFβR expression in cells treated with higher doses of imatinib confirmed the inhibition of receptor activation (I.P.: immunoprecipitation; I.B.: immunoblotting).