2D and 3D Self-Assembling Nanofiber Hydrogels for Cardiomyocyte Culture
Figure 5
Cardiomyocytes on different nanofiber coatings stained positively using ((a)–(e), (g)–(k)) cardiac antibodies or ((f), (l)) LIVE/DEAD Kit. Red is ((a)–(e), (g)–(k)) troponin T, green is (g) MLC2v or ((h)–(k)) MHC, and blue is DAPI (nuclei). NRCs grew as well on top of nanofiber coatings (b) 1 (), (c) 1 + HyA (), and (d) 3 () as on (a) the control (untreated commercial well plate, ), whereas on (e) 2 + HyA () they did not attach well and formed clusters. Cardiomyocytes differentiated from hESCs grew well on top of nanofiber coatings (h) 1 + HyA () and (i) 4 () and fairly well on nanofiber coating (j) 5 () when compared to (g) the control (commercial well plate coated with 0.1% gelatin, ). On nanofiber coating (k) 6 (), the cells did not grow on top of the coating, but they surrounded it. The round structure () is a particle of the coating. The ratio between living and dead cells was approximately the same on every coating with both (f) NRCs and (l) hESC-CMs. The same scale bar (200 μm) applies to every image.
