285740.fig.002
Figure 2: Purification result of recombinant protein. Lane Ms: protein molecular weight marker; Lane1: crude cell extracts of uninduced E. coli Rosetta containing pET-30a; Lane 2: crude cell extracts after 4 h past the induction without IPTG of E. coli Rosetta containing pET-30a-His-DS; Lane3: crude cell extracts after 4 h past the induction with IPTG of E. coli Rosetta containing pET-30a-His-DS. Lane4: the purified recombinant protein of DS. The purified molecular mass fused with His-tag protein agree well with those predicted for the fusion proteins.