Protein Coexpression Using FMDV 2A: Effect of “Linker” Residues
Figure 7
Location of key substitutions that affect 2A cleavage activity. Amino acid sequence comparison of parental pGFP-F2A20-CherryFP (F2A20wt) and pGFP-F2A18-CherryFP (F2A18wt) and their corresponding mutant derivatives (F2A20mut and F2A20Fm, F2A18mut, and F2A18Fm) used to coexpress GFP and CherryFP proteins from a single ORF (F2A sequence in italics). Mutated residues are boxed, and cleavage activities are indicated by “−” or “+”.
