A New Protocol to Detect Multiple Foodborne Pathogens with PCR Dipstick DNA Chromatography after a Six-Hour Enrichment Culture in a Broad-Range Food Pathogen Enrichment Broth
Figure 2
DNA strip for chromatography (a) and reaction results (b) for three pathogens and a negative control. (a) Line 1 (Tag1): EHEC, Line 2 (Tag2): Shigella spp. and EIEC, Line 3 (Tag3): Salmonella spp., Line 4 (Tag4): internal control, Line 5: flow control. (b) Strains: no. 1 Eschesrichia coli O157 (shiga toxin 1 and 2) GTC14510; no. 2 Shigella dysenteriae O1 (shiga toxin 1 and IpaH) GTC01057; no. 3 Salmonella enterica serovar Enteritidis GTC03838; and no. 4 internal positive control. All of amplicons are reacted with each tag.
