A New Protocol to Detect Multiple Foodborne Pathogens with PCR Dipstick DNA Chromatography after a Six-Hour Enrichment Culture in a Broad-Range Food Pathogen Enrichment Broth

Table 1

List of bacterial strains used in this study.

Bacteria	Serotype	Toxin	Strains	Number of strain
EHEC; (Enterohemorrhagic E. coli)	O26: H-	Shiga toxin 1	GTC14538, GTC14548, GTC14605, GTC14606	4
	O26: H11	Shiga toxin 1	GTC14516, GTC14540, GTC14549, GTC14557, GTC14558	5
		Shiga toxin 1 and 2	GTC14515, GTC14539, GTC14567	3
	O111: H-	Shiga toxin 1	GTC14517, GTC14528, GTC14541	3
		Shiga toxin 1 and 2	GTC14508, GTC14582	2
	O115: H10	Shiga toxin 1	GTC14518	1
	O119: H2	Shiga toxin 1	GTC14529	1
	O121: H19	Shiga toxin 2	GTC14530, GTC14577, GTC14601, GTC14602	4
	O128: H-	Shiga toxin 1 and 2	GTC14603	1
	O157: H7	Shiga toxin 2	GTC14513, GTC14514,GTC14524, GTC14525, GTC14537 GTC14546, GTC14547, GTC14550, GTC14553, GTC14560	10
		Shiga toxin 1 and 2	GTC14510, GTC14511, GTC14512, GTC14521, GTC14535 GTC14536, GTC14544, GTC14545, GTC14551, GTC14552,	10
	O157: H-	Shiga toxin 1 and 2	GTC14507, GTC14520, GTC14530, GTC14543, GTC14555 GTC14556, GTC14566, GTC14571, GTC14587, GTC14588	10
EIEC; (Enteroinvasive E. coli)	O28: H-		GTC14240, GTC14243, GTC14251, GTC14259, GTC14260	5
	O124: H-		GTC14241, GTC14242, GTC14245, GTC14262	4
	O136: H-		GTC13248, GTC14254	2
	O144: H-		GTC14249, GTC14252, GTC14256	3
	O164:H-		GTC14244, GTC14246, GTC14247,	3
Salmonella enterica
subsp.enterica	serovar Typhimurium		GTC02557, GTC02561, GTC02562, GTC02563, GTC02564	10
			GTC02571, GTC02572, GTC-2573, GTC02574, GTC02575
subsp.enterica	serovar Enteritidis		GTC03838, GTC00131, GTC02377, GTC02382, GTC02387	7
			GTC02389, GTC02390
subsp.enterica	serovar Dublin		GTC13214, GTC13215, GTC13216, GTC13217,GTC13218	10
			GTC13219, GTC13220, GTC13221. GTC02558, GTC02560
subsp.enterica	serovar Typhi		GTC3P001, GTC3P074,GTC3P076, GTC3P081, GTC3P085	10
			GTC3P087, GTC3P091, GTC3P095, GTC3P100, GTC3P106
subsp.enterica	serovar ParaTyphi A		GTC3P002, GTC3P082, GTC3P083	3
Salmonella bongori			GTC03793T	1
Shigella boydii			GTC00779T, GTC01912, GTC01913, GTC01914, GTC01915	8
			GTC01915, GTC01916, GTC01917,
Shigella dysenteriae			GTC01057T, GTC00786, GTC01929, GTC01930, GTC14808	17
			GTC14809, GTC14810, GTC14811, GTC14812, GTC14813,
			GTC14814, GTC14815, GTC14816, GTC14817, GTC14818,
			GTC14819, GTC14820,
Shigella flexneri			GTC 0780T, GTC01918, GTC01920, GTC02007, GTC02008	13
			GTC02009, GTC02010, GTC02011, GTC02012, GTC02013,
			GTC02015, GTC02016, GTC02014,
Shigella sonnei			GTC00781T, GTC01909, GTC01910, GTC01911, GTC01931	7
			GTC01932, GTC01933,
Escherichia coli			GTC00503 T	1
Escherichia albertii			GTC 16441T	1
Escherichia fergusonii			GTC 01720T	1
Escherichia vulneris			GTC 10613T	1
Escherichia hermannii			GTC 10612T	1
Escherichia blattae			GTC 01342T	1
Citrobacter freundii			GTC 14890T	1
Citrobacter diversus			GTC 00114T	1
Citrobacter rodentium			GTC 14911T	1
Citrobacter youngae			GTC 14914T	1
Klebsiella pneumoniae			GTC 14868T	1
Enterobacter cloacae			GTC 00109T	1
Enterobacter aerogenes			GTC 14962T	1
Cronobacter sakazakii			GTC 14952T	1
Serratia marcescens			GTC 14672	1
Yersinia enterocolitica			GTC 00127T	1
Pseudomonas aeruginosa			GTC 00002T	1
Vibrio parahaemolyiticus			GTC 02055	1
Staphylococcus aureus			GTC 00286T	1

GTC is the Gifu Type Culture Collection supported by the National Bioresource Project (NBRP: http://www.nbrp.jp/) of the Ministry of Education, Culture, Sports, Science,and Technology.
“T” after the strain number means type strain.