A New Protocol to Detect Multiple Foodborne Pathogens with PCR Dipstick DNA Chromatography after a Six-Hour Enrichment Culture in a Broad-Range Food Pathogen Enrichment Broth

Table 2

Cocktail primer list.

Pathogen	Targeted gene	Primer name	Sequence (5′-3′)
EHEC	stx1	Forward stx1	Biotin-ACAGGATTTGTTAACAGGAC
		Reverse stx1	Tag1-TCTGTATTTGCCGAAAACGT
	stx2	Forward stx2	Biotin-GATACAGAGAGAATTTCGTC
		Reverse stx2	Tag1-GCCAGTTATCTGACATTCTG
Shigella spp. and EIEC	ipaH	Forward ipaHF	Biotin-CTCGCAGAGAAACTTCAGCTCT
		Reverse ipaHR	Tag2-TTCTCTTCACGGCTTCTGACCAT
Salmonella spp.	invA	Forward invA	Biotin-TGACAGAATCCTCAGTTTTTCA
		Reverse invA	Tag3-AGATAAGACGGCTGGTACTGAT
Internal control		Forward IPC	Biotin-ACTCTTCCTAGCAGGATCCCTCTAAG
		Reverse IPC	Tag4-GCAATTCTAATACGACTCACTATAGG