A New Protocol to Detect Multiple Foodborne Pathogens with PCR Dipstick DNA Chromatography after a Six-Hour Enrichment Culture in a Broad-Range Food Pathogen Enrichment Broth

Table 3

Detection sensitivity of CPDC assay.

DNA concentration	EHEC serovar O111, GTC14517 (stx1)	EHEC serovar O157, GTC14513 (stx2)	Shigella dysenteriae serovar 2, GTC01929 (ipaH)	Salmonella enterica serovar Enteritidis GTC03838 (invA)
2 ng/assay	+	+	+	+
200 pg/assay	+	+	+	+
20 pg/assay	+	+	+	+
2 pg/assay	+	+	+	+
200 fg/assay	+	+	+	+
20 fg/assay	−	−	−	−

Serially diluted purified DNA of each strain was used to count detection sensitivity.