Defects in Base Excision Repair Sensitize Cells to Manganese in S. cerevisiae

Figure 3

Attenuation of the cytotoxic effect of Mn²⁺ by exogenous antioxidants. Sensitive strain ubc13 was treated with 1.5 mM Mn²⁺, 1.5 mM Mn²⁺ plus 10 mM glutathione (GSH), and 1.5 mM Mn²⁺ plus 20 mM N-acetylcysteine (NAC), as described in Section 2. Survival was determined relative to untreated strain (100% survival). Wild-type strain was treated with 2 mM Mn²⁺, with or without cotreatment with GSH and NAC, as described in Section 2. At least 5 independent colonies were tested. Average survival plus standard deviation is shown.