In Vitro Characterization of a Multifunctional Staphylokinase Variant with Reduced Reocclusion, Produced from Salt Inducible E. coli GJ1158
Figure 5
(a) Time course activation of plasminogen by SRH and SAK. The plasminogen activation activities of SRH and SAK were determined as a function of generated plasmin. Plasminogen (5 μM) and SRH or SAK (10 nM) were incubated at 37°C. At different time point, aliquots were withdrawn and assayed for plasmin activity with Spectrozyme PL (0.3 mM). Data represent a mean ± S.D. of three independent experiments performed under same condition. (b) Antihrombin activity of recombinant protein SRH and SAK. The Antithrombin activity was expressed as inhibition of amidolytic activity of thrombin. Thrombin (200 nM) activity was estimated with Spectrozyme TH (0.3 mM) at the final concentration of 150 μM of SRH or SAK. Data represent a mean ± S.D. of three independent experiments performed under same condition. There are significant statistical differences of antithrombin activity between SRH and SAK ().
