In Vitro Characterization of a Multifunctional Staphylokinase Variant with Reduced Reocclusion, Produced from Salt Inducible E. coli GJ1158
Figure 6
Time course of turbidimetric fibrin clot and platelet-rich clot lysis assay. Fibrin clot or platelet-rich clots were incubated with HBS or with recombinant protein SRH or SAK (100 nM) in a solution containing 1.5 μM plasminogen. The decrease in with time was used to calculate the relative clot turbidity at different time point. The experiments were repeated for three times and data represent the means ± S.D. (a) Platelet-rich clot lysis assay: of SAK and of SRH were found 226.98 ± 4.62 min and 76.54 ± 4.74 min, respectively, at the final concentration of 100 nM. Control clots treated with HBS only showed that readings were stable throughout the incubation period. (b) Fibrin clot lysis assay: The of SAK (186.70 ± 2.76 min) and of SRH (191.46 ± 6.65 min) were not significantly different at the final concentration of 100 nM.
